Calcium/Calmodulin-dependent Protein Kinase Activates Serum Response Factor Transcription Activity by Its Dissociation from Histone Deacetylase, HDAC4
Davis, F., Gupta, M., Camoretti-Mercado, B., Schwartz, R., and Gupta, M. Calcium/Calmodulin-dependent Protein Kinase Activates Serum Response Factor Transcription Activity by Its Dissociation from Histone Deacetylase, HDAC4. The Journal of Biological Chemistry Vol. 278, Issue 22, 20047-20058, (2003).
“Protein A/G-agarose (20µl) was then added to each sample and incubated at 4°C overnight on a rotating device.”
”...reactions were continued at room temperature with continuous rocking for an additional 1 h.”
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Genomic Analysis of Stationary-Phase and Exit in Saccharomyces cerevisiae: Gene Expression and Identification of Novel Essential Genes
Martinez, M., et al. Genomic Analysis of Stationary-Phase and Exit in Saccharomyces cerevisiae: Gene Expression and Identification of Novel Essential Genes. Molecular Biology of the Cell Vol. 15, 5295-5305 (2004).
“Before microarray printing, ...were incubated at 21°C in 48-52% humidity for 4-20 h...”
”Slides were incubated in single-slide capacity chambers...on a rotating platform at 42°C for 16-20 h.“
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Mechanical Strain on Osteoblasts Activates Autophosphorylation of Focal Adhesion Kinase and Proline-rich Tyrosine Kinase 2 Tyrosine Sites Involved in ERK Activation
Boutahar, N., Guignandon, A., Vico, L., and Lafage-Proust, M. Mechanical Strain on Osteoblasts Activates Autophosphorylation of Focal Adhesion Kinase and Proline-rich Tyrosine Kinase 2 Tyrosine Sites Involved in ERK Activation. The Journal of Biological Chemistry Vol. 279, Issue 29, 30588-30599, (2004).
“After incubation at 4 °C for 2 h on a rotating device, immune complexes were precipitated at 4 °C overnight on a rotating device by using protein-A Sepharose...”
“After transfer, membranes were blocked using 5% nonfat dried milk in PBS, pH 7.4, and incubated overnight at 4 °C with the...”
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Role of bone marrow cell trafficking in replenishing skeletal muscle SP and MP cell populations
Rivier, F. et al. Role of bone marrow cell trafficking in replenishing skeletal muscle SP and MP cell populations. Journal of Cell Science Vol. 117, 1979-1988 (2004).
“Muscles were finely minced using sterile scalpels and digested at 37°C in 1U ml-1 dispase (Roche) and 2 mg ml-1 collagenase-type 4 (Worthington) for 45 minutes using an Enviro-Genie...”
“100 ng of denatured probe were added to each slide and sections were hybridized overnight at 37°C in a humidified chamber.”
“Slides were incubated in a humidified chamber at 37°C for 30 minutes with digoxigenin blocking solution... then for an additional 30 minutes with...”
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Screening lambda cDNA or genomic libraries
Koelle, M. 1997, “Screening lambda cDNA or genomic libraries”, Yale University. Available from: http://info.med.yale.edu/mbb/koelle/protocols/protocol_lambda_library_sc.html.
“Add the probe to the blot/hyb. Solution, mix well, and incubate overnight at 65° on a rocker...”
“Place on a rotating platform at room temp for 1-2 hours.”
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Structure — function studies of human apolipoprotein A-V: a regulator of plasma lipid homeostasis
Jennifer A. Beckstead, Michael N. Oda, Dale D. O. Martin, Trudy M. Forte, John K. Bielicki, Trish Berger, Robert Luty, Cyril M. Kay, and Robert O. Ryan. Structure — function studies of human apolipoprotein A-V: a regulator of plasma lipid homeostasis” Biochemistry, 42 (31), pp 9416 - 9423 (2003).
“Subsequently, the IgG fraction was biotinylated by incubation for 15 min with 5 ml 0.5 mg/ml sulfo-NHS-Ssbiotin... in borate buffer on a rocking platform with slow agitation.”
“Non specific binding sites on the membrane were blocked with 0.1% TTBS...overnight, at room temperature while rotating.”
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Subtraction of cap-trapped full-length cDNA libraries to select rare transcripts
Hirozane-Kishikawa, T. et al. Subtraction of cap-trapped full-length cDNA libraries to select rare transcripts. BioTechniques Vol. 35, No. 3, 510-518 (2003).
“One microliter of 5 M NaCl was then added to the driver/tester mixture, and the hybridization was performed at 4°C for 8 h...”
“The hybridized sample library was diluted with 500 µL HAP buffer, incubated at 65°C, and added to the HAP, mixed vigorously, and rotated at 60°C for 30 min.”
“The HAP was washed twice with 500 µL HAP buffer, rotating at 60°C for 30 min...”
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