“Culture for 20 h with shaking in a deep-well, conical-bottom microplate.” (Yeast Culture protocol)

“Shake at 800 rpm for 10 min at 30°C.” (ß-Galactosidase Assay)

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“We pipetted 10 µl bead aliquots in a 96-well plate, adding 5 µl of water plus 5 µl of PCR product per well. After mixtures were incubated for 3 h at 22 °C, with mixing at 800 r.p.m, …”

“We incubated the plate for 45 min at 22 °C with mixing at 800 r.p.m …”

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“Pipet 90 µl NaR-Assay Buffer-NADH solution (prepared in Step 8 of Reagent Preparation) to each well. Shake on a plate mixer for ~20 minutes @ 800 rpm.”

“Add 50 µl Color Reagent No. 1 and 50 µl Color Reagent No. 2 solution to each well. Shake on a plate mixer for ~10 minutes @ 800 rpm.”

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“[sterile 2-mL, 96-deep-well, round-bottom blocks are] grown at 900 rpm and 37°C.”

“…cultures are allowed to shake at 900 rpm and 37°C for an additional 4-5 h.”

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