Culture of Human Cervical Epithelial Cells

Stanley, M., 2002, ‘Culture of Human Cervical Epithelial Cells’, Culture of Epithelial Cells, Second Edition, Freshney, R., Freshney, M. (eds.), Wiley-Liss, Inc.: New York pp. 137-169.

“Place the universal on a magnetic stirrer in an incubator or hot room at 37°C and stir slowly for 30-40 min.”

Determination of Hydrogen Peroxide (H2O2) Residual in Fiber Matrices

Determination of Hydrogen Peroxide (H2O2) Residual in Fiber Matrices, Solvay Chemicals, Inc., 2004, Houston, Texas.

“2. Add 50mL of water and a small magnetic stir bar to the flask containing the sample. 3. Agitate for 15 minutes using a magnetic stirring device. Make sure the slurry is agitated sufficiently to stay suspended during this period. 4. Turn off the stirrer. 5. Add 20 mL of... Swirl to mix. 6. Add 25 ml of... Swirl to mix. 7. Let the slurry stand for 5 minutes... 8. Resume stirring.”

“12. Turn off the stirrer and let the slurry stand for 2.0 hours... 13. Resume stirring and titrate as in “11.” above.

Method 9213: Potentiometric Determination Of Cyanide In Aqueous Samples and Distillates With Ion-Selective Electrode

United States Environmental Protection Agency. “Method 9213: Potentiometric Determination Of Cyanide In Aqueous Samples And Distillates With Ion-Selective Electrode” EPA Publication SW-846: Test Methods For Evaluating Solid Waste, Physical/Chemical Methods. Washington: GPO, 1996.

“Add a PTFE-coated magnetic stir bar, place the beaker on a magnetic stir plate, and stir at slow speed (no visible vortex).

Preparation and characterisation of chitosan and trimethyl-chitosan modified PCL nanoparticles as DNA carriers

Haas, J. et al. Preparation and characterisation of chitosan and trimethyl-chitosan modified PCL nanoparticles as DNA carriers. AAPS PharmSciTech. 2004.

“As for the aqueous phase, 100 mg PVA and 15 mg CS or TMC were stirred in 5 ml water for 2 hours at room temperature until a clear solution was obtained. The organic phase was passed through a 0.22µm syringe filter to remove any un-dissolved solids and subsequently added drop-wise to the aqueous phase under constant stirring. The resulting micro-emulsion was kept under constant agitation on magnetic stirrer at 1,000 rpm for 1 hour...”

“This colloidal preparation was diluted to a volume of 50 ml by adding water drop wise under stirring conditions (1,000 rpm, magnetic stirrer), which resulted in nanoprecipitation.”

Synthesis of (-)-(E,S)-3-(Benzyloxy)-1-Butenyl Phenyl Sulfone via a Horner-Wadsworth-Emmons Reaction of (-)-(S)-2-(Benzyloxy) Propanal

‘Synthesis of (-)-(E,S)-3-(Benzyloxy)-1-Butenyl Phenyl Sulfone via a Horner-Wadsworth-Emmons Reaction of (-)-(S)-2-(Benzyloxy) Propanal’, Organic Syntheses, Collective Volume 10, Freeman, J. (ed.), Wiley: New York, p. 66; Vol. 78, p. 177, 2004.

“The reaction mixture is stirred for 48 to 60 hr at room temperature (Note 7) and subsequently diluted with water (100mL) and hexane (300 mL). Stirring is continued for an additional 3 hr at room temperature.”

“...is added very slowly dropwise to the solution of the ester and stirring is continued for at least 1 hr after the complete addition of the DIBAH solution (Note12). Upon complete consumption of the ester, the crude reaction mixture is poured directly with vigorous stirring into 360 mL of...”

“The aldehyde solution is added dropwise at 0°C with vigorous stirring. After complete addition the reaction mixture is stirred for ca. 12 hr and allowed to warm...”